TTIB-196 U266B1 人外周淋巴細胞

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ATCC® Number: TIB-196™ Price: $269.00

Designations: U266B1 [

U266]

Depositors: NK Nilsson

Isotype: IgE; lambda light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens （human）

Morphology: lymphoblast

TTIB-196 U266B1 人外周淋巴細胞

Source: Organ: peripheral blood

Disease: myeloma; plasmacytoma

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; interleukin 6 （interleukin-6, IL-6）

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Applications: transfection host （Nucleofection technology from Lonza）

DNA Profile （STR）: Amelogenin: X,Y

CSF1PO: 12,13

D13S317: 12

D16S539: 10

D5S818: 11,12

D7S820: 11,12

THO1: 5,7

TPOX: 8

vWA: 17

Age: 53 years adult

Gender: male

Comments: U266 cells have been reported to produce human IL-6.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1180: Nilsson K, et al. Established immunoglobulin producing myeloma （IgE） and lymphoblastoid （IgG） cell lines from an IgE myeloma patient. Clin. Exp. Immunol. 7: 477-489, 1970. PubMed: 4097745

22377: Kawano M, et al. Autocrine generation and requirement of BSF-2/IL-6 for human multiple myelomas. Nature 332: 83-85, 1988. PubMed: 3258060

32396: Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

TTIB-196 U266B1